Time & Location
Feb 7 - March 8, 2012
Tuesday and Thursday
FHCRC Campus, Weintraub Bldg.
Grading is based:
- 50% on presentation (lit review) and class participation during other people's presentations.
Each student will be assigned one research paper to present. Presentations should be timed for 20 min maximum, picking on the major points. Do not feel that you need to explain every panel of every figure. All students must read each paper. and are expected to come to class ready to discuss when called on. This means reading the paper in order to understand it, and looking up references if there are points you do not understand.
- 50% on your written assignment
The written assignment is a 2-page Nature style "mini-review" on any recent paper(s) on cell migration. The papers should not be one of the papers discussed in lit review and review approval by Jon or Cecilia. The assignment is due on Tues March 13.
Class Schedule: Week 1
Tue Feb 7: Introduction - Cecilia
Cells that migrate and fundamentals of migration; actin polymerization, force and traction
Thur Feb 9: Molecules and machines of cell migration - Jon
General principles: Self-organization, cooperativity, catch bonds, conformation changes, mechanosensing
Featuring: actin; Arp2/3, WAVE and formins; Rac1 and PIP2; integrins; talin and Cas
Tue Feb 14: literature review. Two papers about the importance of Rac in cell migration in vitro.
A "classic": Ridley et al, Cell. 1992 Aug 7;70(3):401-10. PMID:1643658, and a newer paper applying a neat tool for manipulating Rac activity: Wu et al, Nature 2009 Sep 3;461(7260):104-8. PMID:19693014
Thur Feb 16: literature review. Two papers about cancer cell migration in 3D.
Cancer cells use invadopodia to penetrate the ECM: Eckert et al. Cancer Cell 2011 Mar 8;19(3):372-86. PMID:21397860
How matrix stiffness affects 3D cell migration: Levental et al, Cell 2009 Nov 25;139(5):891-906. PMID:19931152
Week 3: Polarity and direction sensing in cell migration - Jon
Tue Feb 21: General principles: Polarity vs guidance; chemotaxis; "Local excitation": Positive feedback at the leading edge; "Global inhibition": Inhibition at sides and rear; The Par complex and Cdc42; Microtubules and trafficking
Thur Feb 23: literature review: two papers about cell polarity in epithelial cells in culture. These papers are less "dense" than those assigned last week and hopefully will be more accessible to read and understand.
Yan et al (2012) Dev Cell 22:92 PMID:22264729. A recent paper about how cAMP regulates the actin cytoskeleton in Dictyostelium. ElmoE as a hub linking Gbg, Arp2/3, actin and Rac1.
Dow et al (2007) Oncogene 26:2272. PMID: 17043654. This paper shows the importance of Scribble, an apical polarity gene, in polarizing migrating epithelial cells in vitro.
Week 4: Directed cell migration in development - Cecilia
Tue Feb 28: primordial germ cells, growth cones; instructive and permissive cues
Thur March 1: literature review
Paper #1 (Qing) PMID: 20434207: Tcherkezian et al. (2010) Transmembrane Receptor DCC Associates with Protein Synthesis Machinery and Regulates Translation. Cell 141:632
Paper #2 (Nate) PMID:19098902: Ziel et al (2009) Unc-6 (netrin) orients the invasive membrane of the anchor cell in C. elegant. Nature Cell Biology 11: 183-9.
Paper #3 (Carissa) PMID:20010816: Kardash et al., (2010) A Role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo. Nature Cell Biology 12: 47-53.
Week 5: Collective cell migration - Cecilia
Tue March 6: convergent extension, neural crest, lateral line, border cells
Thur March 8: literature review
Paper #1 (Joseph) PMID:22169071: Weber et al., (2012) A Mechanoresponsive Cadherin-Keratin Complex Directs Polarized Protrusive Behavior and Collective Migration. Developmental Cell 22:104-15
Paper #2 (Chad) PMID:22326025: Majumder et al. (2012) Par-1 Controls Myosin-II Activity through Myosin Phosphatase to Regulate Border Cell Migration. Curr. Biol. Feb.8 (Epub).
Paper #3 (Chelsea) PMID:122215812: Zhang et al., (2012) Phactr4 Regulates Directional Migration of Enteric Neural Crest through PP1, Integrin Signaling, and Cofilin Activity. Genes and Development 26:69-81
If you're off-campus (either UW or FHCRC), click on the appropriate links in this box below before trying to access full text course readings.
Once you've logged in, use your "back" button to return to this page and click on the links to the readings.
FHCRC/MCB students (sign in with your FHCRC library card login and password)
UW students (sign in with your UW NetID login)